World’s oldest semen still viable
Semen stored since 1968 in a laboratory in Sydney has been defrosted and successfully used to impregnate 34 Merino ewes, with the resulting live birth rate as high sperm frozen for just 12 months.
“This demonstrates the clear viability of long-term frozen storage of semen. The results show that fertility is maintained despite 50 years of frozen storage in liquid nitrogen,” said Associate Professor Simon de Graaf from the Sydney Institute of Agriculture and School of Life and Environmental Sciences at the University of Sydney.
“The lambs appear to display the body wrinkle that was common in Merinos in the middle of last century, a feature originally selected to maximise skin surface area and wool yields. That style of Merino has since largely fallen from favour as the folds led to difficulties in shearing and increased risk of fly strike,” Associate Professor de Graaf said.
His colleague on this project, Dr Jessica Rickard, said: “We believe this is the oldest viable stored semen of any species in the world and definitely the oldest sperm used to produce offspring.”
Out of 56 ewes inseminated, 34 were successfully impregnated. This compares to recently frozen semen from 19 sires used to inseminate 1048 ewes, of which 618 were successfully impregnated. This gives a pregnancy rate of 61 percent for the 50-year-old semen against 59 percent for recently frozen sperm, a statistically equivalent rate.
The original semen samples were donated in the 1960s from sires owned by the Walker family. Those samples, frozen in 1968 by Dr Steven Salamon, came from four rams, including ‘Sir Freddie’ born in 1963, owned by the Walkers on their then property at Ledgworth.